Fig. 1
From: Venoarterial extracorporeal membrane oxygenation induces early immune alterations
VA-ECMO induced increased in immature circulating neutrophils. a Total neutrophils were determined by blood count formula at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 9 Control (Ctrl) group, n = 9 VA-ECMO group). Mature and immature peripheral blood neutrophil counts were determined by flow cytometry at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 8 Control (Ctrl) group, n = 9 VA-ECMO group). b Surface marker of neutrophil degranulation (CD35, CD63, CD66b) was compared by flow cytometry at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 8 Ctrl group, n = 9 VA-ECMO group). Data presented as ratio of mean fluorescence intensity ratio (rrMFI), defined as the ratio of rMFI between D0 and D1 or D4. C. Expression of C5a receptor on mature and immature neutrophils compared by flow cytometry at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 8 Ctrl group, n = 9 VA-ECMO group). Data expressed as rMFI