Fig. 5

Effects of mitochondrial transplantation on apoptosis and mitochondrial fusion and fission. a TUNEL assay of the white pulp of the spleen (top). Scale bars, 100 μm. The numbers of apoptotic cells in the spleen were determined (bottom). **p < 0.01 compared with the sepsis group. b Intrinsic apoptosis markers in the spleen were measured by immunoblotting 24 h after induction of sepsis. Cleaved caspase 3, n = 4 per group; pro-caspase 9 and cleaved caspase 9, n = 3 to 8 per group; Cytochrome c, n = 4 to 9 group; BAX, n = 4 to 9 per group; BCL-2, n = 4 to 9 per group. ***p < 0 .001, **p < 0 .01 and *p < 0.05 compared with the sham or sepsis group. c Mitochondrial dynamics markers in the spleen were measured by immunoblotting 24 h after induction of sepsis (n = 7 per group for each marker). *p < 0.05 compared with the sepsis group. MT mitochondria, TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling