Table 4 List of primers for quantification of mRNA expression of mitochondrial quality mechanisms, mitochondrial complexes and oxidation pathways by RT-qPCR

PINK1

Forward:

GACGCTGTTCCTCGTTATGA

Mitophagy

Reverse:

TCCAGCTCCACAAGGATGTT

PARKIN

Forward:

GACCCTCAACTTGGCTACTC

Mitophagy

Reverse:

CTTCGCAGGTGACTTTCCTC

MFN2

Forward:

CCGCCACATAGAGGAAGGAC

Fusion

Reverse:

CGCACAGACACAGGAAGGAG

DRP1

Forward:

AAGCTGCTGCCATAGTCCTC

Fission

Reverse:

ACCACAGCCATGTCAGTGTC

NRF2

Forward:

GCTACTAATCAGGCTCAGTC

Mitochondrial biogenesis

Reverse:

GTAGTCTCAACCAGCTTGTC

TFAM

Forward:

CATGGACTTCTGCCAGCATA

Mitochondrial biogenesis

Reverse:

AGAACACCGTGGCTTCTACA

mnSOD

Forward:

ACGCGGCCTACGTGAACAAC

Antioxidant enzyme

Reverse:

CAACAGATGCAGCCGTCAGC

OGG1

Forward:

GTGTGCGACTGCTGCGACAA

mtDNA damage repair (excision of 8-oxoguanine)

Reverse:

CTGGATGAGCCGAGGTCCAA

HIF1α

Forward:

TGAGGGGACAGGAGGATCAG

Master regulator of cellular and systemic homeostatic response to hypoxia

Reverse:

CACGCGGAGAAGAGAAGGAA

SIRT1

Forward:

ATGCTGGCCTAATAGAGTGG

Regulates epigenetic gene silencing, biogenese and antioxidant mechanisms

Reverse:

TCTGGAACATCAGGCTCATC

NGAL

Forward:

GGTGAGCACCAACTACAACC

Early biomarker of acute kidney injury

Reverse:

GCCCAGAGATTTGGAGAAGC

NDUFA1

Forward;

ACTGGCTACTGCGTACATCC

ETC complex 1 (nDNA)

Reverse:

AGATGCGCCTATCTCTTTCC

COX5b

Forward:

CATTGGCTCCTTCTCCCATA

ETC complex 4 (nDNA)

Reverse:

CATACCAGGTGGTCCCATTC

B-actin

Forward:

AGGATGCAGAAGGAGATCAC

Housekeeping gene

Reverse:

AGTCATAGTCCGCCTAGAAG