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Fig. 3 | Critical Care

Fig. 3

From: A physiological approach to understand the role of respiratory effort in the progression of lung injury in SARS-CoV-2 infection

Fig. 3

Variation of gene expression in high strain and low strain regions of the lung in a murine model of patient self-inflicted lung injury. a Representative images of in vivo/ex vivo fit between tomographic maps of regional strain and 3D digitized frozen lungs. Red areas represent high strain regions, while the green/blue areas represent low strain regions in spontaneous breathing. Low and high strain regions from the same frozen lung were cut, homogenized, and the RNA purified. b Gene expression variation of inflammation/pathological mechanotransduction between regions of high and low regional strain. The genes that increased their expression in regions of high deformation were TNF Superfamily Member 13b (Tnfsf13b, > 8 times), Interleukin-2 receptor subunit beta (Il2rb, > 6 times), Phosphodiesterase 4A (Pde4A, ~ 3 times), 5-hydroxytryptamine receptor 3A (Htr3a), Plasma kallikrein (Klkb1), and Leukotriene C4 Synthase (Ltc4s). These genes are involved in apoptosis, IL-2 signaling, G-protein signaling, activation of ligand-activated ion channels, coagulation, and inflammation, respectively

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