Fig. 3

Functional inference of circRNAs identified in monocytes (CD14), CD4, CD8 T cells, and B cells (CD19) of CAP patients and healthy subjects. a–d Heatmaps showing expression patterns of differentially altered circRNAs (nominal p value < 0.05) in monocytes (a), CD4+ T cells (b), CD8+ T cells (c), and B cells (d) of CAP patients relative to healthy subjects. e Pathway analysis of the host genes producing the circRNAs for monocytes and CD4+, CD8+ T cells. The vertical axis represents the pathways, and the horizontal axis represents the -log10-transformed Benjamini-Hochberg (BH) adjusted p values. Red bars represent pathways for host genes of upregulated circRNA, whereas blue bars denote pathways for host genes of downregulated circRNA in CAP relative to health. Vertical bars depict the cell type, that is, monocytes (orange), CD4+ (green), and CD8+ (cyan). f Cumulative distribution function (CDF) showing the abundance of predicted micro (mi) RNA binding in the exons of differentially abundant circRNAs of monocytes (orange), CD4+ (green), CD8+ (cyan) T cells, and B cells (purple) and human host gene exons that do not circularize (controls, black)