Fig. 1

Detection and characterization of circRNA in monocytes (CD14), CD4, CD8 T cells, and B cells (CD19) of CAP patients and healthy subjects. a Schematic illustration of linear RNA splicing and the back-spliced junction-read leading to the identity of exonic circRNA. Alu, Arthrobacter luteus restriction endonuclease repeats. b Principal component analysis (PCA) plot of all detected circRNAs for monocytes, CD4, CD8 T cells, and B cells (CD19). c Linear RNA and circRNA expression indices for each peripheral mononuclear cell type. d Number of detected circRNAs per gene in monocytes, CD4+, CD8+ T cells, and B cells. e Number of circRNAs that contained different number of exons derived from parental genes in monocytes, CD4, CD8 T cells, and B cells. f Box plot showing log2-transformed (log2) exon length of back-spliced exons in each cell type relative to the genome-wide exon length. g Box plot depicting log2-transformed intron lengths of introns flanking circRNA back-spliced exons for monocytes (CD14), CD4, CD8 T cells, and B cells (CD19) and genome-wide lengths. h Barplot showing the percentage of repetitive elements in introns flanking circRNAs of each cell type. Alu, Arthrobacter luteus restriction endonuclease repeats