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Fig. 3 | Critical Care

Fig. 3

From: Platelet-derived exosomes promote neutrophil extracellular trap formation during septic shock

Fig. 3

Characterization of exosomes isolated from the plasma of septic shock patients. a Electron micrograph of exosomes (indicated by white arrows) derived from the plasma of septic shock patients. Scale bar, 200 nm. b Plasma exosome size distribution was measured by NanoSight tracking analysis. c CD63, TSG101, and GAPDH protein expression in exosomes were quantified by western blot loaded with equal amounts of exosome protein (40 μg). d Exosomes were detected by CD63 expression using flow cytometry. MFI, mean fluorescence intensity. e Protein quantification of exosomes derived from 800 μL plasma from healthy controls (n = 10) and septic shock patients (n = 12) by BCA. f Immunofluorescence images showing PMNs incubated with Dil-labeled exosomes (red) for 2 h. Nuclei were counterstained with Hoechst (blue). Scale bar, 5 μm. g Platelet-derived exosomes were identified by CD41-FITC staining and detected by flow cytometry. The proportion of CD41-positive exosomes was calculated (n = 3). SSC, side scatter. Graphs represent means ± SEM; **P < 0.01 compared within two groups

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