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Fig. 5 | Critical Care

Fig. 5

From: Multiple cross displacement amplification-a more applicable technique in detecting Pseudomonas aeruginosa of ventilator-associated pneumonia (VAP)

Fig. 5

Sensitivity of the MCDA assays using serially diluted P. aeruginosa genomic DNA. aP. aeruginosa genomic DNA was serially diluted to 10 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg, and 1 fg per microliter. When the dilution was more than 100 fg/uL, the green color by MG and the laddering P. aeruginosa pattern by agarose gel electrophoresis were directly observed. The LoD of the PA MCDA assay was as low as 100 fg per microliter (white arrow). b Real-time turbidity was applied to analyze the amplification products. Genomic DNA levels > 100 fg per reaction produced positive reactions

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