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Fig. 4 | Critical Care

Fig. 4

From: Multiple cross displacement amplification-a more applicable technique in detecting Pseudomonas aeruginosa of ventilator-associated pneumonia (VAP)

Fig. 4

Specificity for P. aeruginosa detection by MCDA assays. Of all 77 pure genomic templates, only the genomic DNAs from the P. aeruginosa strains generated positive results. The color shift in the PA-MCDA tubes (tubes 1–17) was directly observed as a green color. A gray color was seen in tubes 18–34, in which 18–21 were E. coli. Tubes 22–23 were S. aureus, of which tubes 24–25 were A. baumannii and tubes 26–34 were respectively S. epidermidis, S. capitis, Streptococcus pyogenes, S. agalactiae, E. faecalis, S. typhimurium, K. pneumoniae, E. cloacae, and S. maltophilia. A 2% agarose gel electrophoresis assay was applied to detect P. aeruginosa MCDA; lane 0, DL 100-bp DNA marker; lanes 1–17 were positive MCDA products that corresponded to P. aeruginosa tubes 1–17; and lanes 18–34 were negative and corresponded to tube 18–34 respectively

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Critical Care

ISSN: 1364-8535