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Fig. 3 | Critical Care

Fig. 3

From: Multiple cross displacement amplification-a more applicable technique in detecting Pseudomonas aeruginosa of ventilator-associated pneumonia (VAP)

Fig. 3

Optimal reaction temperature for the PA-MCDA primer assay. MCDA reactions when detecting the P. aeruginosa gene were monitored by real-time measurement of turbidity. A turbidity of > 0.1 was considered positive. Nine kinetic graphs were obtained at various temperatures (59–67 °C, at 1 °C intervals) with P. aeruginosa DNA at a concentration of 1 ng per tube. The graphs showed that 65 °C was an optimal temperature for amplification

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Critical Care

ISSN: 1364-8535