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Fig. 3 | Critical Care

Fig. 3

From: Enhancer polymorphism rs10865710 associated with traumatic sepsis is a regulator of PPARG gene expression

Fig. 3

The rs10865710 risk allele disrupts transcription factor CREB2 binding. a EMSA with biotin-labeled probes containing either the C or the G allele of rs10865710, incubated with THP-1 cell nuclear extract. The arrow indicates an allele-specific band that interacts with nuclear protein. 10× and 200× indicate 10- and 200-fold unlabeled probes excess over labeled probes. “+” and “−” mean added and unadded, respectively. b Super-shift EMSA of rs10865710. Two independent experiments were performed with similar results. Ab, antibody; C, C allele; G, G allele

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Critical Care

ISSN: 1364-8535