Fig. 2

Cytokine secretion of iNKT cells activated by α-GalCer loaded on PBMCs from BI patients. iNKT cells isolated from a healthy donor were stimulated by PBMCs loaded with α-GalCer from 10 healthy volunteers (HV) (white boxes) or from BI patients (gray boxes), 10 with pneumonia and 9 without pneumonia. Cytokines secretions were analyzed by ELISA after 48 h. a IFN-γ. b IL-13. Data are shown as the concentration of cytokines in pg/ml and are representative of two independent experiments. Negative controls indicate cytokine secretion in the absence of α-GalCer. Right panels show the data obtained for BI patients when split into those who declared pneumonia and those who did not. Top and bottom whiskers represent the extreme values; boxed area represents the 25th percentile, the median, and the 75th percentile (***p < 0.001; statistical analysis, Mann-Whitney test)