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Fig. 6 | Critical Care

Fig. 6

From: Exosomes from endothelial progenitor cells improve outcomes of the lipopolysaccharide-induced acute lung injury

Fig. 6

miRNA-126-3p and miRNA-126-5p target PIK3R2, HMGB1, and VEGFα and regulate tight junction protein expression levels in human small airway epithelial cells (SAECs). To verify RNA sequencing data (Tables 1 and 2), SAECs were transfected with either miR-126-3p mimic, miR-126-5p mimic, or control miRNA for 48 h. PIK3R2, the target of miR-126-3p (a), epithelial tight junction claudin1 (b), claudin4 (c), and occludin (d) mRNA levels were measured by RT-qPCR. HMGB1 (e) and VEGFα (f), the targets of miR-126-5p; epithelial tight junction claudin1 (g); and claudin4 (h) mRNA levels were determined by RT-qPCR. GAPDH served as an internal control. *p < 0.05 compared with the control group; the experiments were performed at least three independent times. Results are represented as mean ± SE

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