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Fig. 1 | Critical Care

Fig. 1

From: TCR activation mimics CD127lowPD-1high phenotype and functional alterations of T lymphocytes from septic shock patients

Fig. 1

Cytokine production in T cells of patients with septic shock compared with those of healthy volunteers (HVs). Intracellular interleukin-2 (IL-2), tumor necrosis factor alpha (TNFα), and interferon gamma (IFNγ) staining was performed after 3 hours of T-cell activation using phorbol 12-myristate 13-acetate (PMA)/ionomycin in whole blood samples from patients with septic shock at day 1 (D1, n = 14), day 3 (D3, n = 18), and day 7 (D7, n = 10) after the onset of shock in comparison with HVs (n = 18). The percentages of cells producing each cytokine (IFNγ: left panel, IL-2: middle panel, and TNFα: right panel) are represented among CD4+ (a) and CD8+ (b) T cells. The percentages of polyfunctional cells simultaneously producing the three cytokines (c) or none of the three cytokines (d) are represented among CD4+ (left panel) and CD8+ (right panel) T cells. Data are presented as Tukey boxplots. Mann–Whitney tests were used to compare values between patients with septic shock and HVs, *P <0.05, **P <0.01

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