Fig. 3

Macrophage subtypes in sepsis with acute kidney injury. Using specific antibodies (Additional file 2: Table S3: Primary antibodies) and scored according to Table 2A,: total macrophages (CD68) and macrophage subtypes (type I: IRF-5, type II: CD163) were immunohistochemically detected and quantified in the glomeruli in kidney samples from patients with sepsis and controls. a Representative picture of a tubular region of a biopsy from a patient with sepsis to illustrate the morphology of an intermediate macrophage. Black arrow: type II macrophage in the peritubular capillary (CD163-positive). White arrow: “intermediate” macrophage with double staining positive for IRF-5 (type I macrophage) and CD163 (type II macrophage) in the peritubular capillary (b). *Statistically significant differences. Black lines are medians T, tubulus