Fig. 5
From: Ventilator-induced lung injury is aggravated by antibiotic mediated microbiota depletion in mice
Microbiota depletion prior to mechanical ventilation had no impact on composition and recruitment of bronchoalveolar lavage (BAL) innate alveolar cells and blood leukocyte populations upon ventilation. Mice received antibiotic broad-spectrum therapy (ATB). Control (Ctrl) mice did not receive antibiotic treatment. Ventilator-induced lung injury was induced 3 days after completing the antibiotic treatment protocol by high tidal volume (HTV) ventilation (34 ml/kg; positive end-expiratory pressure = 2 cmH2O, 4 h). Additional groups of mice were ventilated with low tidal volume (LTV) or did not receive ventilation (NV). a Numbers of BAL leukocytes and frequencies of alveolar macrophages (alvMs), polymorphonuclear cells (PMNs) and inflammatory macrophages (iMs) Ly6Chigh in BAL as quantified by flow cytometry. b Numbers of blood leukocytes and platelets and frequencies of PMNs (granulocytes minus eosinophils) and monocytes measured in EDTA-blood by Scil Vet abc hematology analyzer. Values are given as mean and SD. In a leukocyte numbers, n = 7 (NV ctrl, NV ATB, LTV ctrl, HTV ATB), n = 6 (HTV ctrl) or n = 3 (LTV ATB); leukocyte frequencies, n = 9 (NV ATB), n = 8–9 (NV ctrl), n = 8 (LTV ctrl, HTV ctrl, HTV ATB), or n = 6–7 (LTV ATB); in b n = 9 (NV ctrl, NV ATB), n = 8 (HTV ATB), or n = 7 (LTV ctrl, LTV ATB, HTV ctrl). Analysis was by two-way analysis of variance/Tukey multiple comparison test, *P < 0.05