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Fig. 5 | Critical Care

Fig. 5

From: Ventilator-induced lung injury is aggravated by antibiotic mediated microbiota depletion in mice

Fig. 5

Microbiota depletion prior to mechanical ventilation had no impact on composition and recruitment of bronchoalveolar lavage (BAL) innate alveolar cells and blood leukocyte populations upon ventilation. Mice received antibiotic broad-spectrum therapy (ATB). Control (Ctrl) mice did not receive antibiotic treatment. Ventilator-induced lung injury was induced 3 days after completing the antibiotic treatment protocol by high tidal volume (HTV) ventilation (34 ml/kg; positive end-expiratory pressure = 2 cmH2O, 4 h). Additional groups of mice were ventilated with low tidal volume (LTV) or did not receive ventilation (NV). a Numbers of BAL leukocytes and frequencies of alveolar macrophages (alvMs), polymorphonuclear cells (PMNs) and inflammatory macrophages (iMs) Ly6Chigh in BAL as quantified by flow cytometry. b Numbers of blood leukocytes and platelets and frequencies of PMNs (granulocytes minus eosinophils) and monocytes measured in EDTA-blood by Scil Vet abc hematology analyzer. Values are given as mean and SD. In a leukocyte numbers, n = 7 (NV ctrl, NV ATB, LTV ctrl, HTV ATB), n = 6 (HTV ctrl) or n = 3 (LTV ATB); leukocyte frequencies, n = 9 (NV ATB), n = 8–9 (NV ctrl), n = 8 (LTV ctrl, HTV ctrl, HTV ATB), or n = 6–7 (LTV ATB); in b n = 9 (NV ctrl, NV ATB), n = 8 (HTV ATB), or n = 7 (LTV ctrl, LTV ATB, HTV ctrl). Analysis was by two-way analysis of variance/Tukey multiple comparison test, *P < 0.05

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