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Fig. 3 | Critical Care

Fig. 3

From: Erythrocyte P2X1 receptor expression is correlated with change in haematocrit in patients admitted to the ICU with blood pathogen-positive sepsis

Fig. 3

Freeze-thaw - control experiments. a Whole blood drawn from healthy volunteers was incubated either with vehicle (Veh), 100 μM NF449 (P2X1 antagonist) or 100 μM A804598 (P2X7 antagonist) and frozen and stored for 3 weeks as whole blood at − 80 °C. After thawing, lysis was measured as absorbance of free haemoglobin in supernatant at 540 nm. n = 3, mean ± SEM. b Flow cytometry of thawed whole blood samples diluted 1000-fold, to ascertain equal distribution of blood cell sub-populations. Cells were identified by forward scatter FSC and side scatter SSC and quantified based on fixed regions. c Bar graph shows data presented as mean ± SEM, n = 3. ns, not significant

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Critical Care

ISSN: 1364-8535