Fig. 1
From: Vasculotide reduces pulmonary hyperpermeability in experimental pneumococcal pneumonia
Vasculotide (VT) binds and phosphorylates mouse Tie2. a Fluorescence intensity with the incubation of mouse Tie2Fc receptor and VT (closed circles) or VT alone (open squares). b Relative light units (RLU) of the specific binding of VT to Tie2Fc, which were corrected to the baseline RLU values of VT alone, are shown. The dissociation constant of the normalized specific binding of VT to mouse Tie2Fc was 34.95 nM (95% CI 2.92–66.97 nM). Values were normalized to the bottom plateau (RLU value of 0 nM VT) and the top plateau at saturation. c No saturation of binding is observed with human immunoglobulin G Fc and VT. d Mouse primary lung microvascular endothelial cells were stimulated with 1, 10, 100, and 1000 ng/ml VT and 800 ng/ml human angiopoietin-1 and normalized to the vehicle-treated group. Data represent mean ± SEM (n = 3–6). *p < 0.05, **p < 0.01, ***p < 0.001 by one-way analysis of variance (Dunnett’s post hoc test)