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Fig. 1 | Critical Care

Fig. 1

From: Three-dimensional ultrastructure of capillary endothelial glycocalyx under normal and experimental endotoxemic conditions

Fig. 1

Scanning and transmission electron microscopy showing glycocalyx of continuous capillaries in the heart under normal conditions. a1 Cardiac capillary without lanthanum nitrate staining. a2 Expanded view of the area within the red rectangle square in (a1). Continuous capillaries in the heart have a continuous thin basement membrane. b1 Cardiac capillary with lanthanum nitrate staining. The endothelial glycocalyx, which is the bush-like structure, can be seen on the surface of the vascular endothelium. b2 Backscattered electron microscopic image of the same specimen as in (b1). The location of backscattered electrons is consistent with the bush-like structure. c1 Energy-dispersive spectroscopic image of a cardiac capillary stained with lanthanum nitrate. c2 Ingredient analysis of the area within the red rectangle in (c1). The bush-like structure includes lanthanum, indicating this structure is the endothelial glycocalyx. C Carbon, O Oxygen, P Phosphorus, S Silicon, La Lanthanum. d1 Transmission electron microscopic imaging of cardiac capillary with lanthanum nitrate staining. d2 Expanded view of the area within the red rectangle in (d1). The endothelial glycocalyx can also be seen on the surface of the vascular endothelium

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