Fig. 1

S. aureus (a) and E. coli (b) binding to EC monolayers under flow. S. aureus and E. coli were labelled with fluorescein. Bacterial suspensions were perfused through Ibidi chambers over confluent EC monolayers and timelapse bacterial binding was measured by fluorescence microscopy. Bacteria binding to EC was assessed in the presence or absence of cilengitide. Cilengitide was added at different time points (indicated by arrows): t = 0 s, t = 15 s, t = 30 s and t = 180 s (n = 3–6 for S. aureus and n = 3–11 for E. coli). Asterisks indicate that results are different from control form that point onwards, P < 0.05