Fig. 1

The single-nucleotide polymorphism rs11666254 inhibits lipopolysaccharide (LPS)-induced FPR2/ALX messenger RNA (mRNA) and protein expression. Data are presented as the mean and SD. The whole-blood samples collected from 98 trauma patients were mixed 1:1 (vol/vol) with RPMI 1640 culture medium and incubated with 100 ng/ml of Escherichia coli LPS (O26:B6) at 37 °C for 4 h. (a) FPR2/ALX mRNA and (b) protein expression in the peripheral leukocytes were assayed using quantitative polymerase chain reactions (presented as relative abundance) and flow cytometry (presented as mean fluorescence intensity [MFI]), respectively. One-way analysis of variance was used to assess statistical significance. a For mRNA expression, P = 0.002 for dominant association (GG vs GA + AA) and P = 0.025 for recessive effect (GG + GA vs AA). P = 0.012 for GG vs GA; P = 0.002 for GG vs AA; P = 0.151 for GA vs AA. b For protein expression (MFI), P = 0.007 for dominant association (GG vs GA + AA), and P = 0.035 for recessive effect (GG + GA vs AA). P = 0.029 for GG vs GA, P = 0.004 for GG vs AA, P = 0.163 for GA vs AA. There was no significant difference between genotypes before LPS stimulation