Fig. 2
Modulation of the glucocorticoid receptor and nitric oxide signaling pathways. a Graphical representation of the glucocorticoid receptor (GR) pathway was performed through Ingenuity Pathway Analysis. The relative modulation of gene expression between burn patients and healthy volunteers (HV) is color coded from green (the gene is less expressed in patients with burns) to red (the gene is more expressed in patients with burns). b, c To focus on the effect of hydrocortisone administration, a zoom was performed on the targets of the glucocorticoid receptor, according to analysis 1 (placebo-treated burn patients vs. HV (b)) or analysis 2 (hydrocortisone-treated patients vs. placebo-treated patients (c)). d Summary of the effect of hydrocortisone administration on the gene expression for the nitric oxide mediated signal transduction pathway [GO:0007263] in HV (green), hydrocortisone-treated patients (red) or placebo-treated patients (blue). Statistical significance of the observed differences was assessed using repeated measures analysis of variance taking into account treatment, time and gene (probes) effects. We observed significant modulation over time (p value < 10 -6), and according to hydrocortisone administration (p value < 10 -6). S1 to S4 sample times day 0, day 1, day 5 and day 7, respectively