Fig. 1From: Lice, rodents, and many hopes: a rare disease in a young refugee a A specific real-time PCR, able to detect the secY house keeping gene, was used to detect pathogenic Leptospira species in blood and urine. b The detection of the louse-borne relapsing fever (LBRF) agent was carried out by a species-specific real-time PCR, targeting an internal region of the B. recurrentis/B. duttonii recN gene. The nucleotide sequence analysis of the 16S rRNA multispacer sequence typing (MST) was utilized as a molecular tool for the microbial genotyping of LBRFBack to article page