Fig. 4
From: Impaired Ca2+ release contributes to muscle weakness in a rat model of critical illness myopathy
Immunofluorescent staining of sham-operated (SHAM) rat and ICU rat muscle samples. Longitudinal sections of rat tibialis anterior (TA) muscle stained with antibodies to Na+ channels (a, b) or dihydropyridine receptor (DHPR) (c, d) in SHAM rat and ICU rat muscles. The discrete and regular white punctate staining in SHAM muscle fibers is less obvious in ICU rat muscle fibers. e-f Cross-sections of SHAM rat (e, g) and ICU rat (f, h) muscles stained for ryanodine receptor (RyR) (e, f) or sarco-endoplasmic reticulum Ca2+ ATPase 1 (SERCA1) (g, h) proteins. Majority of SHAM rat muscle fibers show gray or bright staining closer to the sarcolemma. In contrast, staining is less bright and less extensive in ICU rat muscle fibers. Images are representative of three TA muscles from SHAM and ICU rats, respectively. White asterisks (b, d, f, h) indicate fibers that were considered examples of positive staining. Scale bar, bottom right (a-d) represents 20 μm and 40 μm (e-h)