|
Parameter
|
Per muscle wet weight (pmol/s.mg Ww)
|
Per CS activity (pmol.nkat-1.s-1)
|
|---|
|
ICU (n = 7)
|
Control (n = 8)
|
P
|
ICU (n = 7)
|
Control (n = 8)
|
P
|
|---|
|
OXPHOS (3p)
|
7.6 (5.0–8.8)*
|
13.9 (11.3–17.9)
|
<0.01
|
31 (28–36)*
|
37 (32–74)
|
0.15
|
|
RC capacity (3u)
|
8.6 (6.7–10.5)
|
16.4 (13.0–20.6)
|
0.03
|
41 (37–44)
|
42 (37–98)
|
0.46
|
|
Non-mito OCR
|
0.8 (0.6–1.5)
|
0.8 (0.6–1.3)
|
0.91
|
4 (3–5)
|
2 (1–4)
|
0.16
|
|
F1FoATPase
|
Absolute
|
6.1 (4.8–7.6)*
|
12.6 (9.2–13.0)
|
<0.01
|
26 (26–30)*
|
33 (29–49)
|
0.46
|
|
% OXPHOS
|
81 (77–83)*
|
84 (80–89)
|
0.36
|
81 (77–83)*
|
84 (80–89)
|
0.36
|
|
Proton leak
|
Absolute
|
1.3 (1.0–1.4)*
|
2.2 (1.3–3.6)
|
0.10
|
8 (5–9)*
|
7 (4–11)
|
0.95
|
|
% OXPHOS
|
19 (17–23)*
|
16 (11–20)
|
0.36
|
19 (17–23)*
|
16 (11–20)
|
0.36
|
|
Complex I
|
4.8 (4.0–6.1)
|
6.7 (5.5–8.6)
|
0.19
|
23 (22–35)
|
23 (18–26)
|
0.35
|
|
Complex II
|
4.6 (2.9–6,5)
|
1.5 (0.8–3.8)
|
0.06
|
23 (20–28)
|
8 (3–14)
|
<0.01
|
|
Complex III/ GPDH
|
1.5 (1.1–1.9)
|
0.8 (0.4–1.3)
|
0.12
|
7.4 (6.0–9.3)
|
1.8 (1.2–3.9)
|
<0.01
|
|
Complex IV
|
15.5 (13.0–19.5)
|
19.7 (15.3–27.5)
|
0.30
|
88 (69–99)
|
49 (40–113)
|
0.12
|
- Data presented as median (interquartile range), p value as per Mann–Whitney U test. *N = 5 for ICU patients. GPDH glycerol-3-phosphate dehydrogenase, Non-mito OCR non-mitochondrial oxygen consumption rate, OXPHOS oxidative phosphorylation, RC respiratory chain
