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Table 2 Mitochondrial functional indices measured by high-resolution respirometry in homogenates

From: Mitochondrial function in skeletal muscle of patients with protracted critical illness and ICU-acquired weakness

Parameter

Per muscle wet weight (pmol/s.mg Ww)

Per CS activity (pmol.nkat-1.s-1)

ICU (n = 7)

Control (n = 8)

P

ICU (n = 7)

Control (n = 8)

P

OXPHOS (3p)

7.6 (5.0–8.8)*

13.9 (11.3–17.9)

<0.01

31 (28–36)*

37 (32–74)

0.15

RC capacity (3u)

8.6 (6.7–10.5)

16.4 (13.0–20.6)

0.03

41 (37–44)

42 (37–98)

0.46

Non-mito OCR

0.8 (0.6–1.5)

0.8 (0.6–1.3)

0.91

4 (3–5)

2 (1–4)

0.16

F1FoATPase

Absolute

6.1 (4.8–7.6)*

12.6 (9.2–13.0)

<0.01

26 (26–30)*

33 (29–49)

0.46

% OXPHOS

81 (77–83)*

84 (80–89)

0.36

81 (77–83)*

84 (80–89)

0.36

Proton leak

Absolute

1.3 (1.0–1.4)*

2.2 (1.3–3.6)

0.10

8 (5–9)*

7 (4–11)

0.95

% OXPHOS

19 (17–23)*

16 (11–20)

0.36

19 (17–23)*

16 (11–20)

0.36

Complex I

4.8 (4.0–6.1)

6.7 (5.5–8.6)

0.19

23 (22–35)

23 (18–26)

0.35

Complex II

4.6 (2.9–6,5)

1.5 (0.8–3.8)

0.06

23 (20–28)

8 (3–14)

<0.01

Complex III/ GPDH

1.5 (1.1–1.9)

0.8 (0.4–1.3)

0.12

7.4 (6.0–9.3)

1.8 (1.2–3.9)

<0.01

Complex IV

15.5 (13.0–19.5)

19.7 (15.3–27.5)

0.30

88 (69–99)

49 (40–113)

0.12

  1. Data presented as median (interquartile range), p value as per Mann–Whitney U test. *N = 5 for ICU patients. GPDH glycerol-3-phosphate dehydrogenase, Non-mito OCR non-mitochondrial oxygen consumption rate, OXPHOS oxidative phosphorylation, RC respiratory chain