Fig. 6

MFG-E8-derived short peptide 68 (MSP68) inhibits neutrophil infiltration in the liver after cecal ligation and puncture (CLP). The liver tissues from sham, vehicle and MSP68-treated mice were harvested at 20 h after CLP. a Single cell suspensions of leukocytes were stained with allophycocyanin (APC)-anti-mouse Ly-6G and peridinin chlorophyll protein-cyanine 5.5 (PerCP/Cy5.5)-anti-mouse CD11b, followed by flow cytometric analysis. Representative dot plots of surface Ly6G/CD11b expression on gated live liver leukocytes. Numbers adjacent to outlined areas show the percentage of Ly6G⁺CD11b⁺ neutrophils as indicated. The graphs show percentage (b) and total numbers (c) of neutrophils in the liver. d Liver tissues were homogenized and myeloperoxidase (MPO) activity was determined spectrophotometrically. Data are expressed as mean ± standard error of the mean (n = 4–6/group) and compared by one-way analysis of variance and Student–Newman–Keuls test; *P <0.05 versus sham and ^# P <0.05 versus vehicle