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Fig. 5 | Critical Care

Fig. 5

From: Milk fat globule epidermal growth factor-factor 8-derived peptide attenuates organ injury and improves survival in sepsis

Fig. 5

MFG-E8-derived short peptide 68 (MSP68) inhibits neutrophil infiltration in the lungs after cecal ligation and puncture (CLP). The lung tissues from sham, vehicle and MSP68-treated mice were harvested at 20 h after CLP. a Single cell suspensions of leukocytes were stained with allophycocyanin (APC)-anti-mouse Ly-6G and peridinin chlorophyll protein-cyanine (PerCP/Cy5.5)-anti-mouse CD11b, followed by flow cytometric analysis. Representative dot plots of surface Ly6G/CD11b expression on gated live lung leukocytes. Numbers adjacent to outlined areas show the percentage of Ly6G+CD11b+ neutrophils as indicated. The graphs show percentage (b) and total numbers of neutrophils in the lungs (c). d Lung tissues were homogenized and myeloperoxidase (MPO) activity was determined spectrophotometrically. Data are expressed as mean ± standard error of the mean (n = 4–6/group) and compared by one-way analysis of variance and Student–Newman–Keuls test; *P <0.05 versus sham and # P <0.05 versus vehicle

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Critical Care

ISSN: 1364-8535