Fig. 1

MFG-E8-derived short peptide 68 (MSP68) inhibits adhesion of neutrophils. a Schematic diagram showing a series of synthesized peptides flanking the arginine-glycine-aspartate (RGD) sequence of human MFG-E8 including the 5-aa peptide MSP68 with the valine-arginine-glycine-aspartate-valine (VRGDV) sequence. Calcein AM-labeled differentiated HL-60 cells were added to microtiter plates coated with fibronectin (FN) (10 μg/ml) (b) or 10,000 pulmonary artery endothelial cells (PAECs)/well (c) in the presence of vehicle (phosphate buffered saline (PBS)) or an indicated concentration of MSP68 and incubated at 37 °C for 2 h. Adhered dHL60 cells were detected using a fluorescence plate reader at 485 nm/530 nm. The fluorescence values for cells adhered to FN or PAEC in the presence of PBS are designated as 100 % adhesion. Data are expressed as mean ± standard error of the mean obtained from two independent experiments (n = 3/group) and compared by Student’s t test; *P <0.05 versus PBS