Fig. 7

Renal expression of ERα and GPER 24 h after cardiac arrest and cardiopulmonary resuscitation (CA/CPR). Aging is associated with increased renal expression of ERα in female mice and GPER in male mice 24 h after CA/CPR. ERα and GPER expressions were determined in kidney homogenates of young (10–15 weeks) or aged (78–87 weeks) male (a, c, e) or female (b, d, f) mice harvested after 24 h of CA/CPR by Western blotting with subsequent quantification by densitometry. Densitometric analyses for ERα and GPER are shown in (a–d). Western blots including homogenates from all animals (n = 8 or 9 per group) are shown in (e, f). Note the increase in ERα abundance in aged females (b, f) and increase in GPER abundance in aged males (c, e). Data are presented as mean ± standard deviation, n = 8 or 9 per group, *P < 0.05 by unpaired t test. ER estrogen receptor, GPER G protein-coupled estrogen receptor