|
Parameter
|
Group
|
Baseline
|
Start
|
60 min
|
180 min
|
360 min
|
|---|
|
pH
|
NT
|
7.40 (0.04)
|
7.40 (0.01)
|
7.14 (0.03)
|
7.11 (0.05)
|
7.12 (0.06)
|
|
1T
|
7.40 (0.05)
|
7.41 (0.02)
|
7.34 (0.05)a
|
7.18 (0.05)a
|
7.16 (0.07)
|
|
3T
|
7.43 (0.02)
|
7.40 (0.01)
|
7.35 (0.02)a
|
7.39 (0.01)a,b
|
7.16 (0.03)
|
|
PaCO2 (kPa)
|
NT
|
6.6 (1.2)
|
6.0 (0.5)
|
12.3 (1.2)
|
13.5 (1.4)
|
13.8 (1.5)
|
|
1T
|
6.0 (0.6)
|
5.8 (0.3)
|
10.2 (0.9)a
|
14.6 (2.4)
|
15.2 (3.3)
|
|
3T
|
6.3 (0.7)
|
5.9 (0.7)
|
10.6 (0.7)a
|
13.1 (0.5)
|
22.6 (1.7)a,b
|
|
PaO2 (kPa)
|
NT
|
62.6 (12.7)
|
63.7 (7.4)
|
48.1 (12.0)
|
45.2 (12.3)
|
46.4 (11.8)
|
|
1T
|
65.2 (4.8)
|
65.0 (8.6)
|
59.8 (6.3)
|
49.4 (9.0)
|
53.3 (5.0)
|
|
3T
|
55.5 (15.5)
|
56.0 (4.6)
|
49.7 (4.1)
|
32.6 (7.6)§
|
30.0 (7.7)a,b
|
|
Base excess (mEq/ml)
|
NT
|
4.8 (2.2)
|
2.7 (2.3)
|
1.3 (2.2)
|
1.8 (2.8)
|
3.4 (3.2)
|
|
1T
|
3.0 (3.2)
|
2.4 (1.5)
|
13.8 (3.5)a
|
10.9 (3.1)a
|
10.2 (2.1)a
|
|
3T
|
6.2 (2.1)
|
2.4 (3.0)
|
16.0 (0.6)a
|
31.2 (2.2)a,b
|
27.8 (3.1)a,b
|
|
HCO3− (mmol/L)
|
NT
|
28.5 (1.8)
|
27.2 (2.5)
|
29.7 (2.2)
|
30.8 (2.5)
|
32.3 (2.8)
|
|
1T
|
27.0 (2.8)
|
26.7 (1.5)
|
40.1 (3.2)a
|
39.3 (3.2)a
|
38.8 (2.5)a
|
|
3T
|
29.8 (1.9)
|
26.8 (3.1)
|
42.3 (0.9)a
|
58.6 (2.2)a,b
|
57.2 (2.8)a,b
|
|
SaO2 (%)
|
NT
|
98 (0.3)
|
98 (0.2)
|
97 (0.4)
|
97 (0.6)
|
97 (0.6)
|
|
1T
|
98 (0.2)
|
98 (0.3)
|
98 (0.3)a
|
98 (0.2)
|
97 (0.2)
|
|
3T
|
98 (0.8)
|
98 (0.1)c
|
98 (0.2)a
|
98 (0.3)
|
97 (0.8)
|
|
SvO2 (%)
|
NT
|
71 (11)
|
53 (11)
|
63 (6.1)
|
67 (5.8)
|
67 (11)
|
|
1T
|
68 (4.6)
|
43 (5.2)
|
54 (10)
|
65 (6.5)
|
66 (10)
|
|
3T
|
70 (7.6)
|
47 (11)c
|
61 (7.9)
|
69 (4.6)
|
74 (2.1)
|
|
VO2 (ml/min)
|
NT
|
133 (32)
|
124 (22)
|
118 (23)
|
120 (9)
|
121 (21)
|
|
1T
|
128 (28)
|
142 (18)
|
131 (20)
|
139 (14)c
|
140 (26)
|
|
3T
|
133 (30)c
|
123 (18)c
|
117 (7)
|
127 (7)
|
134 (13)
|
|
Qs/Qt (fraction)
|
NT
|
0.15 (0.03)
|
0.11 (0.04)
|
0.17 (0.04)
|
0.20 (0.07)
|
0.20 (0.08)
|
|
1T
|
0.13 (0.03)
|
0.09 (0.02)
|
0.11 (0.03)a
|
0.17 (0.05)c
|
0.15 (0.04)
|
|
3T
|
0.16 (0.02)c
|
0.12 (0.02)c
|
0.15 (0.03)b
|
0.24 (0.04)
|
0.26 (0.06)b
|
|
Lactate (mmol/L)
|
NT
|
1.1 (0.26)
|
1.3 (0.23)
|
0.7 (0.08)
|
0.6 (0.14)
|
0.6 (0.14)
|
|
1T
|
1.6 (0.81)
|
1.4 (0.14)
|
1.1 (0.19)a
|
0.6 (0.14)
|
0.7 (0.19)
|
|
3T
|
1.1 (0.32)
|
1.2 (0.15)
|
0.9 (0.31)
|
0.9 (0.23)a
|
1.3 (0.59)a,b
|
|
ΔPaCO2 (kPa)
|
NT
|
1.5 (0.6)
|
2.2 (0.6)
|
1.9 (0.5)
|
2.2 (0.7)
|
2.1 (0.8)
|
|
1T
|
1.8 (0.4)
|
2.6 (0.3)
|
0.7 (1.1)a
|
2.1 (0.3)
|
2.2 (0.6)
|
|
3T
|
1.5 (0.3)
|
2.7 (0.3)
|
0.6 (0.4)a
|
0.2 (0.6)a,b
|
1.0 (0.7)a,b
|
- Abbreviations: BE arterial base excess, HCO3− arterial bicarbonate concentration, NT control animals that did not receive THAM, PaCO2 arterial carbon dioxide tension, ΔPaCO2 arteriovenous difference in carbon dioxide tension, PaO2 arterial oxygen tension, Qs/Qt venous admixture obtained at inspired oxygen fraction 1.0, SvO2 mixed venous oxygen saturation, 1T group that received THAM infusion for 1 h, 3T group that received THAM infusion for 3 h, THAM Tris(hydroxymethyl)aminomethane, VO2 body oxygen consumption
- The time points refer to time after start of THAM infusion or corresponding time points in the controls (NT)
- aValue is different from NT group
- bValue is different from 1T group
- cOnly 5 subjects represented owing to technical error
