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Fig. 3 | Critical Care

Fig. 3

From: Experimental acute lung injury induces multi-organ epigenetic modifications in key angiogenic genes implicated in sepsis-associated endothelial dysfunction

Fig. 3

RNA polymerase II (Pol II) changes at Angpt1, Tek, Kdr, and Ngal genes in experimental acute lung injury-induced sepsis (ALI-sepsis). (A) Intron-exon organization of genes analyzed in these studies. Exons are shown as rectangles and introns as lines. Chromatin immunoprecipitation platform polymerase chain reaction (ChIP PCR) amplicons are shown as black boxes. (B) ALI was induced in anesthetized mice as in Fig. 1. Mice without any intervention served as controls. After 6 hours of treatment, the lungs, kidneys, and livers were harvested, flash-frozen, and stored at −80 °C. Sheared cross-linked chromatin was prepared from frozen lungs, kidneys, and livers. ChIP analysis was done by using an antibody to the Pol II C-terminal domain (CTD). ChIP DNA samples were analyzed by real-time quantitative PCR with primers to the first and last gene exons (A). Data represent mean ± standard error of the mean (n = 12 animals from each group), expressed as a fraction of input. Statistical differences are shown as in Fig. 2

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Critical Care

ISSN: 1364-8535