Chemerin receptor 23 (ChemR23) in experimental acute lung injury (ALI). Wild-type (WT) and ChemR23 knockout (ChemR23−/−) mice received infusions with NaCl, fish-oil (FO)- or soybean oil (SO)-based lipid emulsions and were subjected to lipopolysaccharide (LPS) for the indicated time points. (a) WT mice receiving FO displayed the lowest leukocytes in their bronchiolar lavage fluid (BALF) at 8 h compared to NaCl (*P <0.05 versus NaCl), whereas the SO group had the highest values (a
P <0.05 versus FO and NaCl). The effect of FO-treatment was diminished in ChemR23−/− showing significantly increased alveolar leukocyte invasion compared to WT (b
P <0.05). After 24 h, the WT-FO group displayed lowest leukocytes counts (c
P <0.05 versus NaCl and SO). At this time point, ChemR23−/− mice of the NaCl and FO group revealed significantly elevated alveolar leukocytes compared to the respective WT (d
P <0.05). (b) Protein extravasation 24 h after LPS challenge in WT animals infused with FO was lowest compared to NaCl and SO (*P <0.05), whereas ChemR23−/− mice showed significantly increased protein leakage in mice infused with NaCl or FO compared to the respective WT controls (b
P <0.05). (c) Macrophage inflammatory protein (MIP)-2 levels 8 h after LPS challenge were significantly elevated in ChemR23−/− mice infused with NaCl or FO compared with the respective WT animals (a
P <0.05). After 24 h the lowest MIP-2 levels were detected in the FO group of WT mice compared to NaCl and SO (*P <0.05).