Figure 3
From: Immunomodulation by lipid emulsions in pulmonary inflammation: a randomized controlled trial
Impact of infusions on monocyte rolling and adhesion to a human endothelial monolayer after LPS inhalation. Isolated monocytes originated from volunteers receiving fish oil (FO)- or soybean oil (SO)-based lipid infusion, or normal saline (NaCl). Adhesion (a) and rolling (b) were investigated on TNF-α-activated endothelial cells under laminar flow conditions (mean ± standard error of the mean; n = 12 for baseline and 5 to 6 for post-inhalation experiments). Adhesion was significantly different 8 h after inhalation between all groups (aFO versus SO, P <0.01; bNaCl versus FO or SO, P <0.05. The increased adhesion in the SO group was significantly different from its baseline and 24-h values (c P <0.05 for each comparison). At 8 h post-inhalation, adhesion in the FO group was lower as compared to its baseline (* P <0.05). Rolling was reduced in all infusion groups 8 h after LPS inhalation and the reduction was significant in the NaCl and FO groups (a P <0.05; * P <0.01 versus respective baseline and P <0.05 versus 24 h). Rolling at 8 h was lower in monocytes isolated after FO infusion as compared to both other groups (b P <0.01 versus SO and P <0.05 versus NaCl).