|
Variable
|
Unit
|
TEG R-time
|
TEG Angle
|
TEG MA
|
FF MA
|
|---|
| | |
β (SE)
|
t
|
P
|
β (SE)
|
t
|
P
|
β (SE)
|
t
|
P
|
β (SE)
|
t
|
P
|
|---|
|
Univariate
| | | | | | | | | | | | | |
|
Syndecan-1
|
100 ng/ml
|
0.82 (0.24)
|
3
|
0.001
|
−3.06 (091)
|
3
|
0.001
|
−3.40 (0.79)
|
−4
|
<0.001
|
−2.00 (0.64)
|
−3
|
0.002
|
|
Thrombomodulin
|
ng/ml
|
0.13 (0.06)
|
2
|
0.021
|
−0.49 (0.22)
|
−2
|
0.026
|
−0.60 (0.19)
|
−3
|
0.002
|
−0.58 (0.15)
|
−4
|
<0.001
|
|
Protein C
|
10%
|
−0.20 (0.10)
|
−2
|
0.044
|
1.09 (0.37)
|
3
|
0.004
|
1.49 (0.32)
|
5
|
<0.001
|
1.05 (0.25)
|
4
|
<0.001
|
|
SOFA score
|
point
|
0.30 (0.08)
|
4
|
<0.001
|
−1.25 (0.30)
|
−4
|
<0.0001
|
−1.27 (0.26)
|
−5
|
<0.001
|
-
|
-
|
NS
|
|
D-dimer
|
mg FEU/l
|
0.03 (0.02)
|
2
|
0.044
|
−0.13 (0.06)
|
−2
|
0.026
|
−0.13 (0.05)
|
−3
|
0.011
|
−0.14 (0.04)
|
−4
|
0.001
|
|
Owren type PT
|
10%
|
−0.29 (0.12)
|
−2
|
0.020
|
1.02 (0.46)
|
2
|
0.029
|
1.39 (0.40)
|
4
|
0.001
|
1.80 (0.30)
|
6
|
<0.001
|
|
P-fibrinogen
|
g/l
|
-
|
-
|
NS
|
1.41 (0.47)
|
3
|
0.003
|
2.62 (0.35)
|
7
|
<0.001
|
3.50 (0.18)
|
19
|
<0.001
|
|
Crystalloid
|
100 ml
|
-
|
-
|
NS
|
-
|
-
|
NS
|
-
|
-
|
NS
|
−0.10 (0.04)
|
−2
|
0.022
|
|
Multivariate
| | | | | | | | | | | | |
|
Syndecan-1
|
100 ng/ml
|
0.64 (0.25)
|
3
|
0.013
|
-
|
-
|
NS
|
−1.78 (0.87)
|
−2
|
0.042
|
−0.84 (0.42)
|
−2
|
0.045
|
|
SOFA score
|
point
|
0.23 (0.08)
|
3
|
0.006
|
−1.19 (0.30)
|
−4
|
<0.001
|
−1.07 (0.27)
|
−4
|
<0.001
|
Not included
|
|
D-dimer
|
mg FEU/l
|
-
|
-
|
NS
|
-
|
-
|
NS
|
-
|
-
|
NS
|
-
|
-
|
NS
|
|
Owren type PT
|
10%
|
-
|
-
|
NS
|
-
|
-
|
NS
|
0.96 (0.40)
|
2
|
0.020
|
0.86 (0.23)
|
3
|
0.001
|
|
P-fibrinogen
|
g/l
|
Not included
|
1.26 (0.44)
|
3
|
0.004
|
-
|
-
|
NS
|
3.44 (0.21)
|
17
|
<0.001
|
|
Crystalloid
|
100 ml
|
Not included
|
Not included
|
Not included
|
-
|
-
|
NS
|
|
Multivariate
| | | | | | | | | | | | | |
|
Thrombomodulin
|
ng/ml
|
-
|
-
|
NS
|
-
|
-
|
NS
|
-
|
-
|
NS
|
-
|
-
|
NS
|
|
SOFA score
|
point
|
0.28 (0.08)
|
2
|
<0.001
|
−1.19 (0.30)
|
−4
|
<0.001
|
−1.24 (0.26)
|
−5
|
<0.001
|
Not included
|
|
D-dimer
|
mg FEU/l
|
-
|
-
|
NS
|
-
|
-
|
NS
|
-
|
-
|
NS
|
-
|
-
|
NS
|
|
Owren type PT
|
10%
|
−0.26 (0.12)
|
−2
|
0.031
|
-
|
-
|
NS
|
1.27 (0.38)
|
3
|
<0.001
|
0.54 (0.20)
|
3
|
0.007
|
|
P-fibrinogen
|
g/l
|
Not included
|
1.26 (0.44)
|
3
|
0.004
|
-
|
-
|
NS
|
3.35 (0.21)
|
17
|
<0.001
|
|
Crystalloid
|
100 ml
|
Not included
|
Not included
|
Not included
|
-
|
-
|
NS
|
|
Multivariate
| | | | | | | | | | | | | |
|
Protein C
|
10%
|
-
|
-
|
NS
|
-
|
-
|
NS
|
1.24 (0.31)
|
4
|
<0.001
|
-
|
-
|
NS
|
|
SOFA score
|
point
|
0.28 (0.08)
|
4
|
<0.001
|
−1.19 (0.30)
|
−4
|
<0.001
|
−1.09 (0.26)
|
−4
|
<0.001
|
Not included
|
|
D-dimer
|
mg FEU/l
|
-
|
-
|
NS
|
-
|
-
|
NS
|
-
|
-
|
NS
|
-
|
-
|
NS
|
|
Owren type PT
|
10%
|
−0.26 (0.19)
|
−2
|
0.031
|
-
|
-
|
NS
|
-
|
-
|
NS
|
0.54 (0.20)
|
3
|
0.007
|
|
P-fibrinogen
|
g/l
|
Not included
|
1.26 (0.44)
|
3
|
0.004
|
-
|
-
|
NS
|
3.35 (0.21)
|
17
|
<0.001
|
|
Crystalloid
|
100 ml
|
Not included
|
Not included
|
Not included
|
-
|
-
|
NS
|
- Regression coefficients (β) with standard errors (SE), t and P values displayed for the univariate and backwards selection multivariate models. Individual backwards multivariate linear regression models including only univariate significant variables, were applied for each investigated biomarker (syndecan-1, sTM and protein C). P values are shown in bold for endothelial-derived biomarkers with P <0.05. Predicted change in TEG R-time (minutes), TEG angle (degrees),TEG MA (maximum amplitude, mm) or FF MA (maximum amplitude, mm) associated with one unit increase in syndecan-1 (100 ng/ml), thrombomodulin (ng/ml), protein C (10%), SOFA score (point), D-dimer (mg FEU/l), Owren type PT (10%), plasma (p)-fibrinogen (g/l) and crystalloids administered in the 24 hours prior to blood sampling and randomization (100 ml).
