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Figure 1 | Critical Care

Figure 1

From: Effects of inhalation of low-dose nitrite or carbon monoxide on post-reperfusion mitochondrial function and tissue injury in hemorrhagic shock swine

Figure 1

Study protocol and experimental design. In the first bleeding period (baseline (BL) to hemorrhage 60 (H60) the animals were bled at a rate of 20 mL/min. They were then kept at mean arterial pressures (MAP) between 30 and 40 mmHg for 90 minutes (H60 to start resuscitation (R0) by repeatedly hemorrhages if necessary at rates of 60 mL/min. At the end of this period the animals were administered the study drug (nitrite or CO). RO samples were obtained before administration of study drug (shadow). The animals were resuscitated with Hextend™ at a volume equal to the total bleeding amount at a rate of 60 mL/min. They were then kept at MAP equal to or above their baseline MAP using norepinephrine, and mixed venous oxygen saturation (SvO2) >70% using dobutamine throughout a four-hour observation period until scheduled euthanasia.

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