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Figure 5 | Critical Care

Figure 5

From: Human severe sepsis cytokine mixture increases β2-integrin-dependent polymorphonuclear leukocyte adhesion to cerebral microvascular endothelial cells in vitro

Figure 5

Effects of severe sepsis cytokine mixture (SSCM) on adhesion molecule ICAM-1 and VCAM-1 expression in hCMEC/D3. hCMEC/D3 were stimulated for 4 hours with either CCM or SSCM preceding analysis of VCAM-1 and ICAM-1 gene expression using qPCR. Data is presented as gene expression normalized to GAPDH and 18S expression, relative to hCMEC/D3 treated with serum-free VascuLife EnGS-Mv cell culture media (n = 5). CCM-control cytokine mixture; SSCM-severe sepsis cytokine mixture; hCMEC/D3-human cerebral microvascular endothelial cells/D3; ICAM-1 – intercellular adhesion molecule-1; VCAM-1 – vascular cell adhesion molecule-1; 18S-18S ribosomal RNA; GAPDH-glyceraldehyde 3-phosphate dehydrogenase; qPCR- quantitative polymerase chain reaction.

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Critical Care

ISSN: 1364-8535