PMN adhesion to hCMEC/D3 following stimulation with severe sepsis cytokine mixture (SSCM). hCMEC/D3 were grown on laminar flow microchannels and interacted with PMN following stimulation of hCMEC/D3, PMN, or both PMN and hCMEC/D3, with CCM or SSCM. In these experiments, hCMEC/D3 were stimulated with CCM or SSCM for five hours. PMN were stimulated for 10 minutes immediately before interacting them with hCMEC/D3. PMN adhesion to hCMEC/D3 was assessed in the presence of flow (laminar shear stress 0.7 dyn/cm2). n = 7; *P <0.05 as compared to corresponding control cytokine mixture (CCM). CCM-control cytokine mixture; SSCM-severe sepsis cytokine mixture; hCMEC/D3-human cerebral microvascular endothelial cells/D3; PMN-polymorphonuclear leukocytes.