Immunofluorescence of microglia with anti-CD11b. Hippocampi were harvested 6 hours, 1 days and 3 days postsurgery (S) or after mechanical ventilation (MV). (A) through (G) Representative photomicrographs of tissue from only surgical animals and surgical animals treated with 6 hours of MV (pictures shown refer to CA2 region of the hippocampus in tissue. Scale bars =50 μm. (H) Cell counts reveal significant differences between S6h and MV6h at 6 hours and 1 day postsurgery or post-MV. One day after surgery, mice showed significantly lower levels of reactive microgliosis compared with surgical mice treated with 3 hours or 6 hours of MV. Values are mean ± SEM. n =4/group. *P <0.05 compared with group S; †
P <0.05 compared with control group (C).