Figure 1

Histological features of lung fibrosis and hydroxyproline content in lungs. (A) Pathological features of lung fibrosis in rats with acute lung injury induced by sepsis and mechanical ventilation. Collagen deposition (see arrows) was marked in septic, non-ventilated and high-VT ventilated lungs (Masson-Goldner, ×400 magnification). (B) Hydroxyproline content in the lungs. C: healthy, anesthetized, non-ventilated, spontaneous breathing; S: septic, anesthetized, non-ventilated, spontaneous breathing; SLVT + PEEP: septic, ventilated with low tidal volume plus positive end-expiratory pressure (PEEP) of 10 cmH₂O; SHVT: septic, ventilated with high tidal volume. Six animals were quantified in each group. ^¶ P <0.01 vs. S; ^* P <0.01 vs. C; ^*** P <0.0001 vs. SLVT + PEEP. VT, tidal volume.