Effect of the rs842647 polymorphism on lipolysaccharide (LPS)-induced TNF-α production. The whole-blood samples collected from trauma patients (n = 30 for the GG and GA genotype, n = 15 for the AA genotype) immediately after admission were mixed at a 1:1 ratio (vol/vol) with RPMI 1640 culture medium and incubated with 100 ng/mL bacterial LPS at 37°C for 4 hours. TNF-α production was determined by a sandwich ELISA. The data are presented as mean ± SD. One-way analysis of variance was used to assess statistical significance. *P = 0.027 for recessive association (AA versus GG + GA).