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Figure 1 | Critical Care

Figure 1

From: β1-adrenoceptor stimulation promotes LPS-induced cardiomyocyte apoptosis through activating PKA and enhancing CaMKII and IκBα phosphorylation

Figure 1

Caspase 3/7 activity and apoptotic index of cardiomyocytes treated with dobutamine (DOB) and lipopolysaccharide (LPS). (A,B) Adult mouse ventricular myocytes treated with DOB at various doses for 24 hours. (C,D) Adult mouse ventricular myocytes exposed to LPS at various concentrations for 24 hours. (E) Representative confocal images of TUNEL assay (green) of adult mouse ventricular myocytes treated with 0.02 μM DOB or/and 10 ng/ml LPS for 24 hours; all cardiomyocytes were stained with anti-cardiac troponin I (cTnI) antibody (red) and total nuclei with 4', 6-diamidino-2-phenylindole (DAPI, blue). White arrowheads indicate apoptotic cardiomyocytes (scale bar = 20 μm). (F,G) AI and caspase-3/7 activity in cardiomyocytes treated with 0.02 μM DOB or/and 10 ng/mL LPS for 24 hours. Mean ± standard error of the mean (n = 4 to 6). * P <0.05, **P <0.01 compared with the control group; # P <0.05, ## P <0.01 compared with the LPS group.

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