Skip to main content
Figure 8 | Critical Care

Figure 8

From: Neutralization of osteopontin attenuates neutrophil migration in sepsis-induced acute lung injury

Figure 8

Effect of rmOPN on dHL-60 cell migration in vitro. Boyden chamber assay was performed as described in Materials and Methods. (A) A representative image of migrated dHL-60 cells labeled with PI (red fluorescence) on the bottom of the transwell membrane. Cells were observed at × 200 original magnification. (B) Migrated dHL-60 cells were counted in five random microscopic fields per well and averaged in each group. Data are expressed as means ± SEM (n = 4/group) and compared by one-way ANOVA and SNK method (* P <0.05 vs. PBS; # P <0.05 vs. rmOPN). ANOVA, analysis of variance; OPN, osteopontin; PBS, phosphate-buffered saline; PI, propidium iodide; rmOPN, recombinant mouse OPN; SEM, standard error of the mean; SNK, Student-Newman-Keuls.

Back to article page