Figure 5
From: Neutralization of osteopontin attenuates neutrophil migration in sepsis-induced acute lung injury
Assessment of neutrophil infiltration into the lungs after CLP . Lung tissues were collected at 20 h after sham-operation, vehicle and anti-OPN Ab treatment in mice. (A) Representative images of the lung tissue sections with immunostaining against Gr-1 at × 200 original magnification are shown. Arrows demarcate examples of areas of staining Gr-1-positive cells. (B) A graphical representation of Gr-1-positive cells averaged over 10 microscopic fields per animal lung tissues. (C) Myeloperoxidase (MPO) activities in lung tissues were determined spectrophotometrically. Data are expressed as means ± SEM (n = 5 mice/group) and compared by one-way ANOVA and SNK method (* P <0.05 vs. shams; # P <0.05 vs. vehicles). Ab, antibody; ANOVA, analysis of variance; CLP, cecal ligation and puncture; OPN, osteopontin; SEM, standard error of the mean; SNK, Student-Newman-Keuls.