Figure 2

Effect of anti-OPN Ab treatment on plasma levels of organ injury markers and proinflammatory cytokines and chemokine in CLP animals . Sepsis was induced in mice by CLP and anti-OPN Ab or non-immunized IgG control at a dose of 50 μg/mice in 100 μl volumes was injected through the jugular vein. In the vehicle group, 100 μl of PBS was injected in CLP mice via the jugular vein. Blood samples were drawn by cardiac puncture at 20 h of sham-operated, vehicle and anti-OPN Ab-treated mice for measuring (A) ALT, (B) AST and (C) LDH. Similarly, the blood samples collected at 20 h after CLP were measured for (D) IL-6, (E) IL-1β and (F) MIP-2 by ELISA. Data are expressed as means ± SEM (n = 5 mice/group) and compared by one-way ANOVA and SNK method (^* P <0.05 vs. shams; ^# P <0.05 vs. vehicle). Ab, antibody; ALT, alanine aminotransferase; ANOVA, analysis of variance; AST, aspartate aminotransferase; CLP, cecal ligation and puncture; ELISA, enzyme-linked immunosorbent assay; IgG, immunoglobulin G; IL, interleukin; LDH, lactate dehydrogenase; MIP-2, macrophage inflammatory protein 2; OPN, osteopontin; PBS, phosphate-buffered saline; SEM, standard error of the mean; SNK, Student-Newman-Keuls.