Figure 2

Microparticles from resuscitated patients increase expression of intracellular adhesion molecule-1 (ICAM-1) vascular cell adhesion molecule-1 (VCAM-1) on endothelial cells. (A, B) Increased ICAM-1 (left panels) and VCAM-1 (right panels) protein expression after endothelial stimulation with microparticles (black bars on the right in each graph) compared with microparticle-free supernatant from resuscitated patients (gray bars; right), shown by western blot analysis and compared with stimulation with microparticles (black bars in the middle of each graph) and microparticle-free supernatant (gray bars; middle) from healthy subjects. Positive and negative controls are shown on the left in each panel. (C) Increased ICAM-1 (panels on the left; green staining by Cy2) and VCAM-1 (panels on the right; red staining by Cy3) protein expression after human umbilical vein endothelial cell (HUVEC) stimulation with microparticles from resuscitated patients (lower right panels in each illustration), shown by immunocytochemistry, compared with microparticle-free supernatant from resuscitated patients (lower left panels) and negative control (upper left panels): 4,6-Diamidino-2-phenylindole (DAPI) was used for staining of nuclei. (**P <0.001; *P <0.01). CPR, cardiopulmonary resuscitation.