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Table 2 Platelet response to exogenous agonists during sepsis

From: Platelet mitochondrial dysfunction in critically ill patients: comparison between sepsis and cardiogenic shock

  Healthy controls Sepsis P value
n 16 16  
Maximal aggregation (%)    
 With ADP (4 μmol/l) 65 ± 18 30 ± 14 <0.001
 With collagen (2 μg/ml) 80 ± 9 36 ± 17 <0.001
 With U46619 (0.5 μmol/l) 75 ± 19 31 ± 18 <0.001
 With thrombin receptor activating peptide (10 μmol/l) 72 ± 27 21 ± 17 <0.001
First wave of aggregation (%)    
 With ADP (4 μmol/l) 53 ± 13 26 ± 10 <0.001
Secretion (nmol ATP/108 platelets)    
 With ADP (4 μmol/l) 0.20 ± 0.18 0.07 ± 0.10 0.041
 With collagen (2 μg/ml) 0.67 ± 0.23 0.35 ± 0.37 0.002
 With U46619 (0.5 μmol/l) 0.28 ± 0.13 0.12 ± 0.13 0.003
 With thrombin receptor activating peptide (10 μmol/l) 0.45 ± 0.26 0.13 ± 0.21 <0.001
  1. Data reported as mean ± standard deviation. Platelet maximal aggregation and secretion (in response to ADP, collagen, thromboxane A2 analogue U46619 and thrombin receptor activating peptide) and first wave of aggregation (in response to ADP) were measured in healthy volunteers and patients with sepsis (within 48 hours from admission to intensive care). Aggregation is expressed as a percentage, where optical density of unstimulated platelet-rich plasma represents 0% and that of platelet-poor plasma is 100%. Secretion was measured as ATP released in the extracellular space (nmol/108 platelets). A curve obtained by adding a fixed dose of ATP to platelet-poor plasma was used as standard. Please note that the platelet count in platelet-rich plasma of patients was above 100 × 103 platelets/mm3 in all but two cases. Blood fibrinogen levels in patients with sepsis were 522 ± 202 mg/ml (internal reference values: 200 to 400 mg/ml). P values refer to Student’s t test or the Wilcoxon rank-sum test.