Figure 6

Three-dimensional structure of Pseudomonas aeruginosa ExoU. Left: position at 0; right: position horizontally rotated −180°. The phospholipase A₂ catalytic dyad comprises S142 and D344. Loop structures such as the K178 area, S329-D344, G439-F444, and Y619-R682 seem to affect the structure of the catalytic dyad. The proposed ubiquitin-binding domain at the C-terminal of ExoU might comprise a pocket structure from these loops. Images of three-dimensional structures were generated by using the protein structure prediction server RaptorX [72].