Sequence analysis with Vuebox®. A region of interest was drawn (yellow line) in the largest possible area of renal cortex close to the ultrasound probe. The software generates a time intensity curve (in yellow in lower panel). This curve is used to generate contrast-enhanced ultrasound (CEUS)-derived parameters. The green curve corresponds to the overall zone (kidney and surrounding tissues) and is not relevant for analysis. T0 corresponds to the destruction of all microbubbles in the scan plan by an ultrasound flash (increase of ultrasound intensity see text for details). Top right panel shows standard B-mode ultrasound image.