Figure 2
From: Early activation of pro-fibrotic WNT5A in sepsis-induced acute lung injury
Representative western blots for WNT5A/β-catenin pathway stimulated by LPS in human MRC-5 cells. (A) Changes in total WNT5A, cyclin D1, metallopeptidase (MMP)7 and vascular endothelial growth factor (VEGF) proteins following exposure to 100 ng/mL lipopolysaccharide (LPS) stimulation for 18 h. Densitometry analysis of the active form (20 kDa) of MMP7 was normalized to the inactive form (30 kDa) and then normalized to β-actin. (B) Changes in non-phospho Ser33/37/Thr41 β-catenin after 18 h of LPS stimulation. Densitometry was performed on at least three different blots per condition and normalized to the respective loading control (β-actin). Protein expression is expressed as fold-change relative to the respective control vehicle (C). ***P <0.001 versus control vehicle (C).