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Figure 5 | Critical Care

Figure 5

From: The E3 ubiquitin ligase TRIM62 and inflammation-induced skeletal muscle atrophy

Figure 5

Starvation leads to skeletal muscle atrophy in vivo . Six- to eight-week-old male C57BL/6 N mice were subjected to food deprivation for 24 hours (n =6) or 48 hours (n =6), as indicated. Mice fed standard chow (controls, -; n =6) were used as controls. (A) Weights of gastrocnemius/plantaris and tibialis anterior muscles normalized to tibia length are shown. Data are presented as mean ± SEM. ***P <0.001, **P <0.01. Quantitative RT-PCR analyses of Atrogin1 (B) and MuRF1 (C) expression, and immunoblotting of proteins using anti-MuRF1 antibody (D), in gastrocnemius/plantaris and tibialis anterior muscles of control animals (-) and mice deprived of food for 24 hours and 48 hours are shown, as indicated. Glyceraldehyde 3-phosphate dehydrogenase (Gapdh) expression and protein content were used as reference values, and data shown are fold changes compared to controls. Data are presented as mean ± SEM. **P <0.01, *P <0.05. MuRF1, Muscle RING (really interesting new gene) finger-containing protein 1.

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